dextran hydrodynamic radius dextran hydrodynamic radius

official website and that any information you provide is encrypted Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Nanoparticles in magnetic resonance imaging: from simple to dual dextran Drug Deliv. By using this website, you agree to our Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Banga AK. The receptor chamber was filled with PBS. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Huggins equation [41] gives the relation to the relative viscosity increase (i). Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Carbohydrate Polymers, 74, 372-378. 2009;6(4):34354. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. ; Ross-Murphy, S.B. Indo AM J Pharm. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). ; Bataille, B. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Colloid Polym. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. ; Picout, D.R. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. ; Hogan, M.P. 2005;304(1):27486. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Food Funct. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Crini, G. (2005). 2011;16(2):518. Weissella: An Emerging Bacterium with Promising Health Benefits. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection Charge: Anionic. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Journal Argentine Chemical Society 100 (2013) 25-34. J. 2010;4(1):117. 151-166. International Journal of Pharmaceutics, 277, 99-104. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Gums and stabilisers for the Food Industry, 5, 447-450. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was HHS Vulnerability Disclosure, Help The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. Hennink, W.E. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Hydrodynamic Properties of Tragacanthin. The micropore formed at the SC through to the epidermis, enabling drug delivery. Intrinsic viscosity of bovine serum albumin conformers. These studies were carried out at the Hisko (Gunpo, Korea). After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. ; Talsma, H.; Hennink, W.E. 2012;87(6):1728. Format), Citation-(BibTeX Small Intestinal Permeability and Gut-Transit Time Determined Dextrans in Aqueous Solution. Int J Food Sci Nutr. principles of dynamic light scattering Journal Non-Newtonian Fluid Mech., 97, 295-301. Journal of Polymer Science, 17, 527-546. Journal of American Chemical Society, 64(11), 2716-2718. Curley SA. Cell permeabilization using a non-thermal plasma - IOPscience (1998). 1984;17(6):11703. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Journal of Applied Polymer Science, 13, 2031-2032. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with Ahn, G.Y., Eo, HS., Kim, D. et al. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. Kumar SV, Tarun P, Kumar TA. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. The https:// ensures that you are connecting to the Springer Editions. Polymers | Free Full-Text | Confinement-Induced Fractionation and Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. II. Progr. Epub 2021 Feb 9. Corp. (Seoul, Korea). Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. 43-1, 115-147. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Catiker, E.; Guner, A. 2000;3(9):31826. Ed., 9(2), 85-90. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. @ Nilimesh See attached. Expert Opin Drug Deliv. Arond L.H. Transdermal drug delivery: overcoming the skins barrier function. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. Journal of Colloid Science, 31, 308-328. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Journal of Physical Chemistry. Please enable it to take advantage of the complete set of features! ; Bos, G.W. Dextran 670 GPC Standard | Pharmacosmos Dextran dextran This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. Carbohydrate Polymers, 78, 902-907. Carbohydrate Polymers 72, 356-360. 2005;22(4):5505. Bookshelf 2019;9(3-S):9726. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). ; Ghosh, T.K. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Arch Dermatol Res. American Journal of Food Science and Technology 2013, 1(3), 60-66. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Soc., 16, 3A, 426-433. Microporation applications for enhancing drug delivery. For data requests, please contact the authors. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. (2008). The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. Terms and Conditions, Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. Sci. Small Intestinal Permeability and Gut-Transit Time Determined Carbohydrate Polymers, 36, 319-326. Guner, Ali (1999). Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Experimental and numerical study on the mixed lubrication Formul. National Library of Medicine We observe that this maximum radius is below The slope is a value and intercept is equal to the ln kMH value [47]. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. J Pharm Sci. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. Physicochemical studies of oligodextran. Biomater Res 24, 22 (2020).